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Bayesian phylogenetic analyses of WGS knowledge from EPICC cohort ended up executed employing BEAST21. The design we employed was just like the CRC phylogenetic analyses documented before2. We produced an enter XML file for BEAST2 with BEAUti. The model and parameters we utilised are as underneath: as all clones have been sampled concurrently, we set all idea dates as 0; as for the substitution product, we utilized the GTR design and established the Gamma Classification Rely to four; we employed the “Calm Clock Exponential” as clock model; As for prior with the peaceful clock charge indicate, we utilized four.
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De novo mutational signatures extraction was done with SparseSignatures20. This tool adopts LASSO regularisation to Increase the in shape, controlled by a regularisation parameter lambda (λ). It implements a plan depending on bi-cross-validation to estimate the optimal values for both the regularisation parameter λ and the number of signatures.
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